Test Directory

1,25-Dihydroxyvitamin D, Serum

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Updated Test Information:

Test Description
1,25-Dihydroxyvitamin D, Serum
Synonym(s)
1,25-Dihydroxyvitamin D
Test ID
DHVD
General Information
Useful for:
As a second-order test in the assessment of vitamin D status, especially in patients with renal disease
Investigation of some patients with clinical evidence of vitamin D deficiency (eg, vitamin D-dependent rickets due to hereditary deficiency of renal 1-alpha hydroxylase or end-organ resistance to 1,25-dihydroxyvitamin D).
Differential diagnosis of hypercalcemia
Specimen Type
Serum
Specimen Requirements
SST
Stability
Refrigerated (preferred) 7 days
Frozen 28 days
Ambient 7 days
Unacceptable Specimen Conditions
Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK
Methodology
Extraction/Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Estimated TAT
2-4 days
Testing Schedule
Monday-Friday
CPT Code(s)
82652
Minimum Sample Volume
1.2 mL
Reference Range
Males:
<16 years: 24-86 pg/mL
> or =16 years: 18-64 pg/mL
Females:
<16 years: 24-86 pg/mL
> or =16 years: 18-78 pg/mL
Performing Lab
Mayo
LOINC Code(s)
62290-2