Test Directory

Hepatitis B e-Antigen, Serum

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Updated Test Information:

Test Description
Hepatitis B e-Antigen, Serum
Synonym(s)

HBeAg; Hepatitis Be Ag; HBe Ag; HBe antigen; Hepatitis Be antigen; EAG

Test ID
EAG
General Information

Useful for:


Specimen Type

Serum

Specimen Requirements

Serum Gel (SST)

Specimen Collection / Processing Instructions

  • For 24 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

  • Centrifuge and aliquot serum into plastic vial within 24 hours

Required Information

Date of collection is required

Stability

  • Frozen (preferred): 28 days

  • Refrigerated: 7 days

  • Ambient: 24 hours

Unacceptable Specimen Conditions

  • Gross hemolysis

  • Gross lipemia

  • Gross icterus

Limitations

  • Biotin (vitamin B7) is a common ingredient in multivitamins and dietary supplements to enhance hair, nail, and skin growth. Biotin can interfere with the assay performance and cause possible false-negative hepatitis Be antigen (HBeAg) and false-positive anti-HBe results. Patients should be instructed to stop taking such multivitamins and dietary supplements for at least 24 hours prior to blood collection

  • Disappearance of HBeAg or appearance of anti-HBe in serum does not completely rule-out chronic hepatitis B carrier state or infectivity

  • Performance characteristics of this assay have not been established in patients under the age of 2 or in populations of immunocompromised or immunosuppressed patients. This assay is not licensed by FDA for testing cord blood specimens or screening donors of blood, plasma, human cell, or tissue products

  • Performance characteristics have not been established for the following specimen characteristics

    • Grossly icteric (total bilirubin level >20 mg/dL)

    • Grossly lipemic (triolein level >3000 mg/dL)

    • Grossly hemolyzed (hemoglobin level >61 mg/dL)

    • Specimen containing particulate matter



Methodology

Chemiluminescence Immunoassay

Estimated TAT

1-2 days

Testing Schedule

Monday-Saturday

Retention

14 days

CPT Code(s)

87350

Additional Information
Minimum Sample Volume

0.5 mL

Reference Range

Negative

Performing Lab

Mayo

LOINC Code(s)

13954-3