Test Directory

Hepatitis C Virus Genotype, Serum

Print this page

Updated Test Information:

Test Description
Hepatitis C Virus Genotype, Serum
Synonym(s)

HCV Genotype; HCVG; HCV Genotyping

Test ID
HCVG
General Information

Useful for:



  • Determining hepatitis C virus (HCV) genotype (1 to 5) to guide antiviral therapy in patients with chronic hepatitis C

  • Differentiating between HCV subtypes 1a and 1b

  • This assay should not be used as a screening test for HCV infection. It should be performed only on specimens obtained from patients confirmed to have HCV RNA levels in serum of 500 IU/mL or higher.

Specimen Type

Serum

Specimen Requirements

Serum Gel (SST)

Additional Processing Details

  • Centrifuge blood collection tube within 2 hours of collection

  • Transfer serum into aliquot tube.

  • Ship specimen frozen on dry ice only

  • If shipment will be delayed for more than 3 days, freeze serum at ≤ -20 ^C  (up to 42 days) until shipment on dry ice

Stability

  • Frozen (preferred): 42 days

  • Refrigerated: 72 hours

Limitations

  • Specimens should contain a recommended minimum HCV viral load of 500 IU/mL

  • Serum specimens previously submitted to other laboratories for non-microbiology tests are NOT acceptable for add-on test requests, due to possible sample-to-sample carryover from automation used for those tests

  • An "Undetected" or "Indeterminate" hepatitis C virus (HCV) genotype result does not rule-out active HCV infection. Test results should be correlated with routine serologic and molecular-based testing, as well as clinical presentation. Specimens with indeterminate results will be automatically evaluated with the subsequent test HCVGR / Hepatitis C Virus Genotype Resolution, Serum

  • Known cross-reactivity between the assay probes and various HCV genotypes limits the ability of this assay to identify multiple HCV genotypes present in a given specimen. Such cross-reactivity or the actual presence of multiple HCV genotypes in the same specimen may result in an "Indeterminate" or multiple/mixed genotype result.

Methodology

Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) followed by Hybridization with Sequence-Specific,
Fluorescent-Labeled Oligonucleotide Probes

Estimated TAT

1-6 days

Testing Schedule

Monday-Friday

Retention

2 months

CPT Code(s)

87902

Additional Information
Minimum Sample Volume

1.5 mL

Reference Range

Undetected

Reflex Conditions

HCV Genotype Resolution

Performing Lab

Mayo

LOINC Code(s)

32286-7