Laboratory Services

Monoclonal Protein Study, 24 Hour, Urine

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Updated Test Information:

Test Description
Monoclonal Protein Study, 24 Hour, Urine
Synonym(s)

Bence Jones Urine; Heavy Chains Urine; Immunoelectrophoresis, Urine; Immunofixation Electrophoresis (IFE); Immunofixation, Urine; Kappa Chains Urine; Lambda Chains Urine; Light Chains Urine; Paraprotein; Special Protein Studies Urine

Test ID
MPSU
General Information

Useful for:


Specimen Type

24-Hour Urine

Specimen Collection / Processing Instructions

  • Collect urine for 24 hours

  • Aliquot at least 25-mL specimen in plastic, 60-mL urine bottle and at least 1-mL of specimen in plastic, 5-mL tube

  • Label specimens appropriately (60-mL bottle for protein electrophoresis and 5-mL tube for protein, total)

  • The addition of preservative or application of temperature controls must occur within 4 hours of completion of the collection

    • Preferred: Refrigerate

    • Acceptable: Ambient, Frozen, Diazolidinyl Urea, Thymol 



  • See Collection and Transportation for 24-Hour Urine Specimens for multiple collections

Required Information

24-Hour volume is required

Stability

  • Refrigerated (preferred): 14 days

  • Frozen: 5 days

  • Ambient: 24 hours

Unacceptable Specimen Conditions

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Limitations

  • Monoclonal gammopathies are rarely seen in patients younger than 30 years of age

  • Hemolysis may cause a discrete band on protein electrophoresis, which will be negative on immunofixation

  • Penicillin may split the albumin band

  • Radiographic agents may produce an uninterpretable pattern

Methodology

  • PTU: Turbidimetry

  • PEU: Agarose Gel Electrophoresis

  • IFXU: Immunofixation

Estimated TAT

1-3 days

Testing Schedule

Monday-Saturday

Test Includes

  • PTU Protein, Total

  • PEU Protein Electrophoresis

  • IFXU Immunofixation

CPT Code(s)

84156

Additional Information
Minimum Sample Volume

25 mL

Reference Range

PROTEIN, TOTAL


< 229 mg/24 hours
Reference values have not been established for patients <18 years of age.
Reference value applies to 24-hour collection.



ELECTROPHORESIS, PROTEIN



The following fractions, if present, will be reported as a percent of the protein, total.
Albumin
Alpha-1-globulin
Alpha-2-globulin
Beta-globulin
Gamma globulin

Performing Lab

Mayo

LOINC Code(s)

33042-3, 13986-5, 2889-4, 13362-9, 13984-0, 13987-3, 19153-6, 57369-1, 13988-1, 13989-9, 44294-7, 42482-0, 42482-0, 32210-7