Test Directory

Group B Streptococcus

Synonym(s):

Beta Group B, Group B strep, GBS, Beta Strep

Application:

Screening during pregnancy (3537 weeks' gestation) for prevention of GBS disease among newborn infants.

Specimen Type:

Vaginal/rectal swab

Specimen Collection:

A vaginal and rectal collection using the preferred eSwab (one or two swab) collection device. Swabs in Amies or liquid Stuart's transport medium are also acceptable. Do not use gel media.

Collection Procedure:

Using the recommended eSwab or other approved bacterial transport medium, begin by swabbing the lower vagina and the rectum through the anal sphincter. This increases the detection of colonization compared with sampling the cervix or vagina alone according to CDC 2010 recommendations. Replace the swab(s) to the collection device and label the container with the patient's full name and another unique identifier. 

If the patient is allergic to penicillin, please order reflex susceptibility testing on the requisition to confirm the use of an appropriate alternative antibiotic.

Click here to see collection instructions

Required Information:

Complete the Incyte Diagnostics requisition with the patient's demographics and relevant clinical history. Label specimen container with the patient's full first and last name, second unique identifier, and specimen source/site. Note the time of collection.

Specimen Stability:

Room temperature: 24 hours
Refrigerated: 4 days
Frozen (after introduction to Lim broth): 30 days

Unacceptable Specimen:

Unlabeled specimens, specimens in incorrect medium, expired collection devices, specimens subjected to freezing or excessive heat are unacceptable.

Department:

Molecular Pathology

Method:

Real-time polymerase chain reaction (PCR)

Turnaround:

12 business days from receipt

Susceptibility testing for positive GBS on penicillin-allergic patients is an additional 12 business days

Performed:

Monday–Sunday

Retention:

Inoculated Lim broth specimen is retained frozen for 30 days

CPT Code:*

87653
87081 and 87184 - for reflex antibiotic susceptibility testing

Test Code(s):

GBSPCR

Reflex susceptibility testing for penicillin-allergic patients: add free text to AOE Source or check box on paper requisition

*The listed CPT codes are for reference use only. Final coding is based on CPT guidelines and individual circumstances.

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