Laboratory Services

Androstenedione, Serum

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Updated Test Information:

Test Description
Androstenedione, Serum

4-Androstene-3,17-dione, 4-Androstenedione, Delta-4-Androstenedione

Test ID
General Information

Diagnosis and differential diagnosis of hyperandrogenism, in conjunction with measurements of other sex steroids

Diagnosis of congenital adrenal hyperplasia (CAH), in conjunction with measurement of other androgenic precursors, particularly, 17-alpha-hydroxyprogesterone (OHPG), 17 alpha-hydroxypregnenolone, dehydroepiandrosterone sulfate (DHEA-S), and cortisol.

Monitoring CAH treatment, in conjunction with testosterone, OHPG, DHEA-S, and DHEA.

Diagnosis of premature adrenarche, in conjunction with measurement of follicle-stimulating hormone and luteinizing hormone as well as other adrenal and gonadal sex-steroids and their precursors

Container Type

Red Top

Specimen Type


Specimen Requirements

0.6 mL

Specimen Collection / Processing Instructions

Centrifuge and aliquot serum into a plastic vial


Refrigerated (Preferred) - 28 days; Frozen 28 days; Ambient 7 days

Unacceptable Specimen Conditions

Gross hemolysis, gross lipemia, gross icterus


Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Estimated TAT

2 to 5 days

Testing Schedule

Monday through Friday

CPT Code(s)


Reference Range

Premature infants
26-28 weeks, day 4: 92-282 ng/dL
31-35 weeks, day 4: 80-446 ng/dL

Full-term infants
1-7 days: 20-290 ng/dL
1 months - 1 year: <69 ng/dL

Tanner Stages                         Age(years)         Reference Range (ng/dL)
Stage I(prepubertal)               <9.8                                 <51
Stage II                                          9.8-14.5                         31-65
Stage III                                      10.7- 15.4                       50-100
Stage IV                                      11.8-16.2                        48-140
Stage V                                       12.8-17.3                         65-210

Tanner Stages                         Age(years)         Reference Range (ng/dL)
Stage I(prepubertal)               <9.2                                 <51
Stage II                                          9.2-13.7                         42-100
Stage III                                      10.0- 14.4                       80-190
Stage IV                                      10.7-15.6                        77-225
Stage V                                       11.8-18.6                         80-240

*Soldin SJ, Brugnara C, Wong EC, eds. Androstenedione. In: Pediatric Reference Ranges. 4th ed. AACC Press; 2003: 32-34

Males: 40-150 ng/dL
Females: 30-200 ng/dL

For SI unit Reference Values, see

STAT Orderable (Y/N)


Performing Lab


LOINC Code(s)