Laboratory Services

Anti Neutrophil Cytoplasm Panel

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Updated Test Information:

Test Description
Anti Neutrophil Cytoplasm Panel

ANCAG, ANCA-Panel, Atypical ANCA, C ANCA, MPO, P ANCA, PR3, Vasculitis, Wegener's Antigen, UOW1551

Test ID
General Information

Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis.

Container Type

6 mL Red Top or Gold SST Tube

Specimen Type


Specimen Collection / Processing Instructions

Separate serum and refrigerate

Minimum Sample Volume

1mL serum


Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern.

Department (code)



Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies.

Testing Schedule

Monday - Friday

Test Includes

ANCAIF       ANCA, by IF
APR3L          Anti PR3 Level
APR3C         Anti PR3 Comment
AMPOL       Anti MPO Level
AMPOC      Anti MPO Comment

CPT Code(s)

83520x2, 86036

Performing Lab


LOINC Code(s)