Laboratory Services

Anti Neutrophil Cytoplasmic Antibody

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Updated Test Information:

Test Description
Anti Neutrophil Cytoplasmic Antibody

ANCA by IF, ANCA by Immunofluorescence, UOW691

Test ID
General Information

Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis.

Container Type

3 mL blood in RED TOP or SST tube

Specimen Type


Specimen Collection / Processing Instructions

Refrigerate serum

Minimum Sample Volume

0.5mL Serum


Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern.

Department (code)



Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies.

Testing Schedule

Monday - Friday

CPT Code(s)


Reference Range

Negative (NRN)

Effective Date: 4/1/2010

Performing Lab


LOINC Code(s)