Laboratory Services

Barley, IgE, Serum

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Updated Test Information:

Test Description
Barley, IgE, Serum

Barleycorn; Hordeum vulgare

Test ID
General Information

Useful for:

  • Establishing a diagnosis of an allergy to barley

  • Defining the allergen responsible for eliciting signs and symptoms

  • Identifying allergens responsible for allergic disease and/or anaphylactic episode

  • Confirming sensitization prior to beginning immunotherapy

  • Investigating the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens

Specimen Type


Specimen Requirements

  • Preferred: Red Top

  • Acceptable: Serum Gel

Minimum Sample Volume

  • For 1 allergen: 0.3 mL

  • For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace


  • Refrigerated (preferred): 14 days

  • Frozen: 90 days


  • Testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or in patients in whom the medical management does not depend upon identification of allergen specificity

  • Some individuals with clinically insignificant sensitivity to allergens may have measurable levels of IgE antibodies in serum, and results must be interpreted in the clinical context

  • False-positive results for IgE antibodies may occur in patients with markedly elevated serum IgE (>2,500 kU/L) due to nonspecific binding to allergen solid phases.


Fluorescence Enzyme Immunoassay (FEIA)

Estimated TAT

1-3 days

Testing Schedule



14 days

CPT Code(s)


Reference Range
Class IgE (kU/L) Interpretation
0 < 0.35 Negative
1 0.35 - 0.69 Equivocal
2 0.70 - 3.49 Positive
3 3.50 - 17.4 Positive
4 17.5 - 49.9 Strongly Positive
5 50.0 - 99.9 Strongly Positive
6 ≥ 100 Strongly Positive
Performing Lab


LOINC Code(s)


Additional Information