Laboratory Services

BRAF Mutation Analysis

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Updated Test Information:

Test Description
BRAF Mutation Analysis


General Information

Bi-directional sequencing of exon 15 of the BRAF gene, which includes qualitative detection of V600 mutations E, K, D, and others, plus other significant exon 15 mutations.  For solid tumors, tumor enrichment is performed before extraction. Expanded coverage for BRAF exons 11 & 15 is available in the RAS/RAF Panel. Testing is available separately or in combination with HRAS, KRAS, and NRAS in the RAS/RAF Panel. 

Useful in selection of melanoma patients for vemurafenib therapy, for determination of prognosis in thyroid and colon cancers, for predicting response to anti-EGFR therapy in colon cancer, and as aid to diagnosis of hairy cell leukemia.

Specimen Type

FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.

Required Information

Complete the Incyte Diagnostics requisition with the patient's demographics, ordering physician, and a copy of the pathology report.


Use cold pack for transport, making sure cold pack is not in direct contact with specimen. All slides can be packed at room temperature.

Department (code)

Molecular Pathology

Estimated TAT

8 Days


Extracted DNA is kept for  30 days

CPT Code(s)


Performing Lab

NeoGenomics Laboratories