Laboratory Services

CMV Qualitative by PCR, Tissue/Cells

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Updated Test Information:

Test Description
CMV Qualitative by PCR, Tissue/Cells
Synonym(s)

Cytomegalovirus by PCR-Tissue or Cells, PCR-CMV-Tissue or Cells, UOW722

Test ID
CMVQLT
General Information

The detection of CMV by real-time PCR amplification. PCR detects amplicons of the immediate-early (IE) viral protein 1 (IE1, UL123) and Glycoprotein B (gB, UL55) coding regions. Detection of CMV DNA (IU/mL) is achieved by amplifying a standard curve at the same efficiency, consisting of serial dilutions of a known amount of DNA containing the CMV amplicons. Results are reported in International Units per milliliter (IU/mL). The minimum virus level that gives a positive result in 95% of replicates is 20 IU/mL (1.3 log IU/mL).


This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

Container Type

Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM).


Paraffin Tissue: DO NOT SEND ENTIRE BLOCK and DO NOT SEND SLIDES. Slice 3-4 slices that are 5-50 micron thick in sterile tube.


Bone Marrow: 5 mL blood in EDTA (Lavender Top) tube, No Heparin aspirate in syringe.


Swab: In sterile tube/universal transport media (UTM).


Cellular fluid: 1 mL in sterile tube


 

Specimen Type

Tissue Biopsy, Paraffin Tissue, Bone Marrow, Swab or Cellular fluid (e.g. BAL or Washing)

Specimen Collection / Processing Instructions

Bone Marrow: 5 mL bone marrow in EDTA (Lavender Top) tube to Laboratory at room temperature within 24 hour of collection.


ONLY COLLECT MONDAY-THURSDAY AND MUST BE RECEIVED IN THE LAB WITHIN 24 HOURS OF COLLECTION TO BE TRANSPORTED TO UW TO MEET THE 48 HOUR REQUIREMENT


Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Minimum Sample Volume

0.5 mL

Additional Processing Details

Buffy coat: Separate buffy coat from bone marrow within 24 hours of collection, Freeze at -10°C or colder.


Tissue/Cellular fluid/Swab: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Unacceptable Specimen Conditions

Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.

Department (code)

Virology, Eastlake (EVIR)

Methodology

Real-time Polymerase Chain Reaction.

Testing Schedule

Wednesday. Results following Thursday.

Test Includes

CMVQLS      -    CMV Qual Specimen Description
CMVQLR     -    CMV Qual Result
CMVQLI       -    CMV Qual Interpretation

CPT Code(s)

87496

STAT Orderable (Y/N)

No

Performing Lab

UW

LOINC Code(s)