Laboratory Services

EGFR Mutation Analysis

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Updated Test Information:

Test Description
EGFR Mutation Analysis

EGFR, epidermal growth factor receptor

Test ID
General Information

Bi-directional sequencing of exons 18-21 of the EGFR gene for detection of EGFR-activating mutations and TKI resistance mutations (including T790M) in these exons. Tumor enrichment is performed before extraction.

EGFR mutation analysis is recommended in non-small cell lung carcinoma (NSCLC) to detect mutations (commonly L858R and exon 19 deletions) associated with increased sensitivity to EGFR tyrosine kinase inhibitors. Detection of TKI resistance mutations such as T790M in patients being treated with a TKI is useful for planning alternate treatment.

Specimen Type

FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at
5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.

Required Information

Complete the Incyte Diagnostics requisition with the patient's demographics, ordering physician, and a copy of the pathology report.


Use cold pack for transporting block during summer to prevent block from melting. Slides can be packed at room temperature


Molecular Pathology

Estimated TAT

8 Days


Extracted DNA is kept for 30 days

CPT Code(s)


Performing Lab

NeoGenomics Laboratories