Laboratory Services

Estradiol, Serum by LCMS

Print this page

Updated Test Information:

Test Description
Estradiol, Serum by LCMS

Enhanced Estradiol, Extracted Estradiol, 17- Beta Estradiol, Estradiol

Test ID
General Information

All applications that require moderately sensitive measurement of estradiol:

-Evaluation of hypogonadism and oligo-amenorrhea in females
-Assessing ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)
-In conjunction with luteinizing hormone measurements, monitoring of estrogen replacement therapy in hypogonadal premenopausal women
-Evaluation of feminization, including gynecomastia, in males
-Diagnosis of estrogen-producing neoplasms in males and, to a lesser degree, females
-As part of the diagnosis and workup of precocious and delayed puberty in females, and, to a lesser degree, males
-As part of the diagnosis and workup of suspected disorders of sex steroid metabolism (eg, aromatase deficiency and 17 alpha-hydroxylase deficiency)
-As an adjunct to clinical assessment, imaging studies and bone mineral density measurement in the fracture risk assessment of postmenopausal women, and, to a lesser degree, older men
-Monitoring low-dose female hormone replacement therapy in postmenopausal women
-Monitoring antiestrogen therapy (eg, aromatase inhibitor therapy)

Container Type

Red top

Specimen Type


Specimen Requirements

1.2 mL serum collected in a red top.

Specimen Collection / Processing Instructions

1. Centrifuge and remove serum from red blood cells within 2 hours of draw.
2. Aliquot serum to submission container (plastic vial).

Minimum Sample Volume



Refrigerated (preferred): 28 days
Room Temperature: 28 days
Frozen: 28 days

Unacceptable Specimen Conditions

Improper labeling, quantity not sufficient, collected in serum or gel SST


Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Estimated TAT

2-4 days

Testing Schedule

Monday - Saturday

Test Includes


CPT Code(s)


Reference Range File View/Download File
Performing Lab

Mayo Clinic

LOINC Code(s)