Laboratory Services

Fetal Hemoglobin F by Flow Cytometry

Name: Incyte Diagnostics
Address: 13103 E Mansfield Ave, Spokane Valley, Washington, 99216, USA
Telephone: +1-If-you-have-questions-regarding-your-bill-or-would-like-to-make-a-payment,-please-visit-our-Billing-page-by-clicking-"Pay-Online"-at-the-top-of-the-page-For-other-inquiries,-please-call-us-at-509-892-2700

Updated Test Information:

Test Description	Fetal Hemoglobin F by Flow Cytometry
Synonym(s)	HgbF; HbF; Fetomaternal Hemorhage; Fetomaternal Bleed, flow cytometry, blood; Fetal RBC %; Fetal Materal Bleed
Test ID	FETAL HGBF-FLOW
General Information	This test is for the detection of fetal bleed, it should not be used to detect the hereditary persistence of fetal hemoglobin (HPFH) or to detect fetal maternal hemorrhage in a mother with HPFH.
Container Type	EDTA Lavender Blood collection tube
Specimen Type	Whole blood EDTA
Alternate Specimen Type	none
Specimen Requirements	Full tube
Specimen Collection / Processing Instructions	Do not centrifuge. Invert several times to mix. Send whole tube - do not aliquot.
Minimum Sample Volume	1 ml
Pediatric Min. Volume (if applicable)	n/a
Stability	Ambient temperature stablity to 30 hours post collection.
Unacceptable Specimen Conditions	Frozen specimens; gross hemolysis, gross lipemia, gross icterus, clotted samples; improperly identified specimens; tube other than EDTA
Department (code)	Flow
Methodology	Flow Cytometry
Estimated TAT	M-Sat 24 hrs. Sun/holiday STAT by arrangement only.
Testing Schedule	M-Sat 24 hrs. Sun/holiday STAT by arrangement only.
Test Includes	% Hemoglobin F RBCs
Retention	2 weeks
CPT Code(s)	88184; 88185
Reference Range	0.0 - 0.10%
Reflex Conditions	n/a
STAT Orderable (Y/N)	by arrangement
Performing Lab	Spokane Valley Mansfield
Additional Information	In hemolytic disease of the newborn, fetal red blood cells become coated with IgG alloantibody of maternal origin, which is directed against an antigen on the fetal cells that is of paternal origin and is absent on maternal cells. The IgG-coated cells undergo accelerated destruction, both before and after birth. The clinical severity of the disease can vary from intrauterine death to hematological abnormalities detected only if blood from an apparently healthy infant is subject to serologic testing. (1) Pregnancy causes immunization when fetal red blood cells possessing a paternal antigen foreign to the mother enter the maternal circulation, an event described as fetomaternal hemorrhage (FMH). FMH occurs in up to 75% of pregnancies, usually during the third trimester and immediately after delivery. Delivery is the most common immunizing event, but fetal red blood cells can also enter the mother's circulation after amniocentesis, spontaneous or induced abortion, chorionic villus sampling, cordocentesis, or rupture of an ectopic pregnancy, as well as blunt trauma to the abdomen.(2) Rh immune globulin (RhIG, anti-D antibody) is given to Rh-negative mothers who are pregnant with a Rh-positive fetus. Anti-D antibody binds to fetal D-positive red blood cells, preventing development of the maternal immune response. RhIG can be given either before or after delivery. The volume of FMH determines the dose of RhIG to be administered. (1) 1. Roback J, Combs MR, Grossman B, Hillyer C, eds: In: Technical manual. 16th ed. AABB Press; 2008:625-637, 888 2. Mayo Clinic Laboratories. Online Test directory FMB Clinical Information; 2023.
Clone	HbF-1