Test Directory

Hemoglobin Electrophoresis Evaluation, Blood

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Updated Test Information:

Test Description
Hemoglobin Electrophoresis Evaluation, Blood
Synonym(s)

A2 Hemoglobin; Alpha Globin Variant; Alpha Thalassemia; Barts Hemoglobin; Beta Globin Variant; Beta Thalassemia; H Disease; Hemoglobin A2; Hemoglobin Cascade; Hemoglobin Electrophoresis Cascade Level 1; Hemoglobin Molecular studies; Hemoglobin Variant; HGB (Hemoglobin) Electrophoresis; Isoelectric Focusing; Capillary electrophoresis; HPLC; High performance liquid chromatography; Mass Spectrometry; Microcytosis; Sickle cell; Sickling Test; Thalassemia

Test ID
HBEL1
General Information

Useful for: 
Diagnosis and classification of hemoglobin disorders, including thalassemias and hemoglobin variants

Specimen Type

Whole Blood EDTA

Alternate Specimen Type

ACD (solution B), green top (sodium heparin)

Specimen Requirements

Lavender top (EDTA)

Specimen Collection / Processing Instructions

10 mL
Send specimen in original tube. Do not aliquot.

Required Information

Include recent transfusion information.


Include most recent complete blood cell count results.


Metabolic Hematology Patient Information (T810) is strongly recommended. Testing may proceed without this information, however if the information requested is received, any pertinent reported clinical features and data will drive the focus of the evaluation and be considered in the interpretation.


The laboratory has extensive experience in hemoglobin variant identification and many cases can be confidently classified without molecular testing. However, molecular confirmation is always available, subject to sufficient sample quantity (eg, multiplex ligation-dependent probe amplification testing requires at least 2 mLs of sample in addition to protein testing requirements). If no molecular testing or specific molecular tests are desired, utilize the appropriate check boxes on the form. If the form or other communication is not received, the reviewing hematopathologist will select appropriate tests to sufficiently explain the protein findings which may or may not include molecular testing.

Stability

Refrigerated: 7 days

Methodology

HBELI: Consultative Interpretation
HGBCE: Capillary Electrophoresis (CE)
HPLC: Cation Exchange/High-Performance Liquid Chromatography (HPLC)
IEF: Isoelectric Focusing (IEF)
MASS: Mass Spectrometry (MS)
HPFH: Flow Cytometry
UNHB: Isopropanol and Heat Stability
HBEL0: Medical Interpretation

Test Includes

HBELI: Hb Electrophoresis Interpretation
HGBCE: Hb Variant, A2 and F Quantitation,B
HPLC: HPLC Hb Variant, B

CPT Code(s)

83020-Quantitation by electrophoresis
83021-Quantitation by HPLC
82664-Electrophoresis, not elsewhere specified (if appropriate)
83068 (if appropriate)
83789 (if appropriate)
88184 (if appropriate)
83020-26 (if appropriate)

Additional Information
Minimum Sample Volume

1 mL (this volume will limit reflex testing possibilities)
3 mL if multiplex ligation-dependent probe amplification is desired

Reference Range

HEMOGLOBIN A
1-30 days: 5.9-77.2%
1-2 months: 7.9-92.4%
3-5 months: 54.7-97.1%
6-8 months: 80.0-98.0%
9-12 months: 86.2-98.0%
13-17 months: 88.8-98.0%
18-23 months: 90.4-98.0%
> or =24 months: 95.8-98.0%

HEMOGLOBIN A2
1-30 days: 0.0-2.1%
1-2 months: 0.0-2.6%
3-5 months: 1.3-3.1%
> or =6 months: 2.0-3.3%

HEMOGLOBIN F
1-30 days: 22.8-92.0%
1-2 months: 7.6-89.8%
3-5 months: 1.6-42.2%
6-8 months: 0.0-16.7%
9-12 months: 0.0-10.5%
13-17 months: 0.0-7.9%
18-23 months: 0.0-6.3%
> or =24 months: 0.0-0.9%

VARIANT 1
0.0

VARIANT 2
0.0

VARIANT 3
0.0

Reflex Conditions

HPFH: Hb F Distribution, B
MASS: Hb Variant by Mass Spec, B
SDEX: Sickle Solubility, B
IEF: Isoelectric Focusing, B
UNHB: Hb Stability, B
ATHAL: Alpha-Globin Gene Analysis
WASQR: Alpha Globin Gene Sequencing, B
WBSQR: Beta Globin Gene Sequencing, B
WBDDR: Beta Globin Cluster Locus Del/Dup,B
WGSQR: Gamma Globin Full Gene Sequencing
HBEL0: Hb Electrophoresis Summary Interp

Performing Lab

Mayo Clinc