Laboratory Services

Hepatitis B e-Antibody, Serum

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Updated Test Information:

Test Description
Hepatitis B e-Antibody, Serum

Antibody to Hepatitis Be antigen; Hepatitis Be antibody; Anti-HBe; HBe Ab; Hepatitis Be Ab; HEAB

Test ID
General Information

Useful for:

Specimen Type


Specimen Requirements

Serum Gel (SST)

Specimen Collection / Processing Instructions

Patient Preparation: For 24 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins

Minimum Sample Volume

0.5 mL

Required Information

  • Date of collection is required

  • Send separate vials If ordered with HBVQN / Hepatitis B Virus (HBV) DNA Detection and Quantification by Real-Time PCR


  • Frozen (preferred): 28 days

  • Refrigerated: 7 days

  • Ambient: 24 hours

Unacceptable Specimen Conditions

  • Gross hemolysis

  • Gross lipemia

  • Gross icterus


  • Biotin (vitamin B7) is a common ingredient in multivitamins and dietary supplements to enhance hair, nail, and skin growth. Biotin can interfere with the assay performance and cause possible false-negative hepatitis B e-antigen (HBeAg) and false-positive HBe antibody (anti-HBe) results. Patients should be instructed to stop taking such multivitamins and dietary supplements for at least 24 hours prior to blood collection

  • Appearance of anti-HBe in serum does not completely rule-out chronic hepatitis B carrier state or infectivity

  • Performance characteristics of this assay have not been established in patients under the age of 2 or in populations of immunocompromised or immunosuppressed patients. This assay is not licensed by FDA for testing cord blood samples or screening donors of blood, plasma, human cell, or tissue products

  • Performance characteristics have not been established for the following specimen characteristics:

    • Grossly icteric (total bilirubin level of >20 mg/dL)

    • Grossly lipemic (triolein level of >3000 mg/dL)

    • Grossly hemolyzed (hemoglobin level of >124 mg/dL)


Chemiluminescence Immunoassay

Estimated TAT

1-2 days

Testing Schedule



14 days

CPT Code(s)


Reference Range


Performing Lab


LOINC Code(s)


Additional Information