Laboratory Services

Hepatitis C Virus Antibody Confirmation, Serum

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Updated Test Information:

Test Description
Hepatitis C Virus Antibody Confirmation, Serum

Anti-HCV Confirmation; HCV Antibody Supplemental Test; HCVL

Test ID
General Information

Useful for:

  • Confirming the presence of hepatitis C virus (HCV)-specific IgG antibodies in serum specimens that are reactive by HCV antibody screening tests

  • Distinguishing between true- and false-reactive HCV antibody screening test results

  • This test is not intended for use as a hepatitis C virus (HCV) antibody screening test for blood or human cells/tissue donors

  • This assay is not useful for detection of early or acute hepatitis C (<2 months from exposure) as immunocompromised patients may not develop detectable HCV antibodies in blood until 6 months after infection

  • This assay is not useful for differentiating between past (resolved) and chronic hepatitis C

    • To distinguish between past (resolved) and chronic hepatitis C, order HCVQN / Hepatitis C Virus (HCV) RNA Detection and Quantification by Real-Time Reverse Transcription-PCR (RT-PCR), Serum

  • This test is indicated for individuals with reactive hepatitis C virus (HCV) antibody results but undetectable HCV RNA levels

  • For screening of asymptomatic individuals for HCV, order HCSRN / Hepatitis C Virus (HCV) Antibody Screen with Reflex to HCV RNA, PCR, Asymptomatic, Serum

  • For detection of HCV in symptomatic at-risk individuals, order HCVDX / Hepatitis C Virus (HCV) Antibody with Reflex to HCV RNA, PCR, Symptomatic, Serum

Specimen Type


Specimen Requirements

  • Preferred: Serum Gel (SST)

  • Acceptable: Red Top

Minimum Sample Volume

0.1 mL

Additional Processing Details

  • Centrifuge blood collection tube  within 2 hours of collection

  • Aliquot serum into plastic vial.

Required Information

Date of collection is required


  • Frozen (preferred): 30 days

  • Refrigerated: 7 days

Unacceptable Specimen Conditions

Gross hemolysis


  • Performance characteristics have not been established for the following types of specimen:

    • Cadaveric specimens

    • Grossly hemolyzed (hemoglobin >100 mg/dL)

    • Presence of particulate matter


Line Immunoassay (LIA)

Estimated TAT

1-14 days

Testing Schedule



14 days

CPT Code(s)


Reference Range


Performing Lab


LOINC Code(s)


Additional Information