Laboratory Services

HLA-B27, Blood

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Updated Test Information:

Test Description
HLA-B27, Blood

B27; Histocompatibility Antigen; Human Leukocyte Antigen; Leukocyte Antigens; LY27; Spondylitis; W-27 (HLA-B27)

Test ID
General Information

Useful for diagnosis of ankylosing spondylitis, juvenile rheumatoid arthritis, and Reiter syndrome

Specimen Type

Whole Blood

Specimen Requirements

Lavender top (EDTA)

Specimen Collection / Processing Instructions

Send whole blood specimen in original tube. DO NOT ALIQUOT.

Minimum Sample Volume

1 mL

Additional Processing Details

Specimen must arrive within 96 hours of draw


Ambient: 4 days

Unacceptable Specimen Conditions

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.


  • Orders received for both this test and SSO1 / HLA Class I Molecular Phenotype, Blood or DISI / HLA Class I Molecular Typing Disease Association (which provides data on all HLA Class I low-resolution antigens, including B27) will be questioned due to test overlap. This HLA-B27 test is best used alone if a particular disease such as ankylosing spondylitis is under consideration

  • Extreme temperature changes during shipping may alter the specimen making it unacceptable for testing


Flow Cytometry

Estimated TAT

2-6 days

Testing Schedule



7 days

CPT Code(s)


Reference Range

An interpretive report will be provided

Performing Lab


LOINC Code(s)

26028-1, 69052-9

Additional Information