Laboratory Services

Testosterone, Total and Free, Serum

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Updated Test Information:

Test Description
Testosterone, Total and Free, Serum
Synonym(s)

Testosterone, Testosterone Free by Calculation, UOW1044

Test ID
FTTEST
General Information

Do not order for patients <18 years.

Container Type

Lithium Heparin (Lime Green) Top PST & Red Top

Specimen Type

Serum

Specimen Requirements

For albumin and SHBG: 4 mL blood in Lithium Heparin (Lime Green) TOP PST tubeFor testosterone by LC-MS/MS: 4 mL blood in RED TOP

Alternate Specimen Type

For albumin and SHBG: 4 mL blood in GOLD TOP SST, RED TOP, ORANGE TOP SSTFor testosterone by LC-MS/MS: 4 mL blood in LAVENDER TOP

Specimen Collection / Processing Instructions

Red Top and Lavender Top Processing: Centrifuge and aliquot serum or plasma to a plastic vial within 24 hours of collection.
Serum (SST) and Plasma (PST) Separator tubes: Centrifuge within 45 minutes of collection

Minimum Sample Volume

requested: 1 mL serum/plasma
minimum: 0.5 mL serum/plasma

Stability

Refrigerated

Methodology

Chemiluminescence, Beckman DxI instrument (SHBG), dye-binding and spectrophotometry (albumin), and LC-MS/MS (testosterone)

Testing Schedule

Daily

Test Includes

Sex Hormone Binding Globulin - SHBG
Free Testosterone, Calculated - TESTF
Testosterone - TESTMS
Testosterone by LCMS Method - TESTMD

CPT Code(s)

84270, 84403

Performing Lab

UW Laboratory

LOINC Code(s)