Laboratory Services

Varicella Zoster Rapid PCR, Swab or CSF

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Updated Test Information:

Test Description
Varicella Zoster Rapid PCR, Swab or CSF
Test ID
VZVRPD
Performing Lab

Labcorp

General Information

The DiaSorin Simplexa™ Varicella Zoster Virus (VZV) Direct FDA approved assay is a rapid real-time PCR that enables the direct amplification, detection of VZV from both cutaneous and mucocutaneous swab specimens and CSF specimens. A well conserved region of the VZV DNA polymerase gene is targeted to identify VZV in the specimen. Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.

Container Type

Swab in viral or universal transport medium (VTM/UTM) or whole blood

Specimen Type

Lavender-top (EDTA) tube, yellow-top (ACD) tube OR viral/universal transport medium (swab) medium (swab)

Specimen Requirements

 1 mL

Specimen Collection / Processing Instructions

Swabs: Transport specimens on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.


CSF: If CSF has cellular material, centrifuge & aliquot clear CSF. Transport CSF on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.

Minimum Sample Volume

 0.1 mL

Stability

Room temperature

Unacceptable Specimen Conditions

Calcium alginate swabs, they may contain substances that inhibit PCR testing.

Department (code)

Clinical Virology

Methodology

Real-time Polymerase Chain Reaction

Testing Schedule

Monday- Friday 07:00- 21:00, Saturday and Sunday 09:00-16:00

Test Includes

VZVRSD         Varicella Zoster PCR, Specimen Desc
VZVRR            Varicella Zoster PCR, Result
VZVCT            Varicella Zoster PCR, CT Value
VZVRIN         Varicella Zoster PCR, Interpretation

CPT Code(s)

87798

LOINC Code(s)