Laboratory Services

Vitamin B12

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Updated Test Information:

Test Description
Vitamin B12
Synonym(s)

B12, Cobalamin

Test ID
B12
General Information

Vitamin B12 determinations should be performed on serum or plasma samples from fasting patients.

Specimen Type

Serum

Alternate Specimen Type

Na‑heparin, Li‑heparin, K2‑EDTA and K3‑EDTA plasma

Specimen Requirements

SST
Aliquot Tube

Specimen Collection / Processing Instructions

SST - Samples should not be taken from patients receiving therapy with high biotin doses (i.e.greater than 5 mg/day) until at least 8 hours following the last biotin administration. Transfer separated serum or plasma to an aliquot tube

Minimum Sample Volume

1 mL

Pediatric Min. Volume (if applicable)

500 uL

Additional Processing Details

Centrifuge and aliquot serum/plasma from cells within 45 minutes of collection

Stability

2 hours at 15‑25 °C, 48 hours at 2‑8 °C, 56 days at ‑20 °C (± 5 °C).
Stability of serum obtained with separating tubes: 24 hours at 2-8 °C

Unacceptable Specimen Conditions

Hemolyzed samples

Methodology

Electrochemiluminescence immunoassay ECLIA

Estimated TAT

0-2 days

Testing Schedule

Monday-Saturday

CPT Code(s)

82607

Reference Range

232-1245 pg/mL

Performing Lab

Incyte Diagnostics

LOINC Code(s)

2132-9